Molecular Cloning and Characterization of Babesia orientalis Rhoptry Neck 2 BoRON2 Protein

Figure 3

PCR amplification of rhoptry neck 2 gene of Babesia orientalis from gDNA and cDNA and amplification of fragment used for polyclonal antibody production. (a) M: nucleotide marker (15000 bps), line 1: amplification of BoRON2 genomic DNA open reading frame showing a specific band of 4035 bp, and line 2: amplification of cDNA showing to have the same size with genomic DNA. (b) Line 1, the amplicon of product used for polyclonal antibody.

(a)

(b)