Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Diagnosis of Schistosoma mansoni Infection in Faecal Samples

Table 1

Loop-mediated isothermal amplification primer sets for S. mansoni DNA amplification.

Primer set	Primer position	Primer sequence 5′ 3′
Sm1-7	F3	GAT CTG AAT CCG ACC AAC CG
	B3	AAC GCC CAC GCT CTC GCA
	FIP: F1c + F2	AAATCCGTCCAGTGGTTT TTTT
	BIP: B1c +B2	GAAAATCGTTGTATCTCCG; CCGAAACCACTGGACGGA TTTT
		TATTTTTAATCTAAAACAAAC

F3: forward outer primer; B3: backward outer primer; FIP: forward inner primer composed of the F1c and F2 primers connected by a TTTT hinge (bold); BIP: backward inner primer composed of the B1c and B2 primers connected by a TTTT (bold) hinge.