Research Article
“CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica
Table 1
Composition of the culture media, PEHPS, TYI-S-33, and CLUPS.
| Component | Brand | PEHPS [9] | TYI-S-33 | CLUPS |
| Liver and pancreas extract (mL) | In-house | 250 | None | None | Lung and pancreas extract (mL) | In-house | None | None | 250 | Liver infusion broth (g) | BD Difco | None | 10 | None | Casein peptone (g) | BD Bioxon | 10 | 20 | 10 | Ascorbic acid (g) | Sigma Aldrich | 0.2 | 1 | 1 | Cysteine (g) | Sigma Aldrich | 1.0 | 1 | 1.26 | Glucose (g) | Tecnica quimica | 6 | 10 | 6 | K2HPO4 (g) | Baker | 1 | 1 | 1 | KH2PO4 (g) | Monterrey | 0.6 | 0.6 | 0.6 | Sodium chloride (g) | Baker | None | 3.3 | None | Ferric citrate (g) | Sigma Aldrich | None | 0.02 | None | Deionized water | | Up to 1L | Up to 1L | Up to 1L |
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