Characterization of Evolutionarily Conserved Trypanosoma cruzi NatC and NatA-N-Terminal Acetyltransferase Complexes

Figure 1

Expression of TcNaa38/TcNaa30 and TcNaa10/TcNaa15 in trypanosomes. (a) Total proteins from different stages of T. cruzi life cycle, epimastigote midlog (M), epimastigote stationary phase (S), trypomastigote (T), and amastigote (A) were used for western blotting. (b) Cross reaction of anti-TcNaa30 and anti-TcNaa38 against different T. brucei strains. Tb-Gvr (T. brucei GVR strain), Tb-R17 (T. brucei R17 strain), and Tc-Brener epimastigote (T. cruzi CL Brener strain). (c) Developmental stage expression of TcNaa10 and TcNaa15 in CL Brener strain epimastigote (E), trypomastigote (T), and amastigote (A). Purified anti-TcNaa10 and anti-TcNaa15 (1:4000, and 1: 2000 dilutions) were used for western blotting. (d) Cross reaction of anti-TcNaa10 (upper panel) and anti- TcNaa15 (lower panel) against different T. brucei strains. The T. brucei strains used were GVR35 (Gvr), R17 and UTRO 091B (Utr).

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