Characterization of Evolutionarily Conserved Trypanosoma cruzi NatC and NatA-N-Terminal Acetyltransferase Complexes

Figure 7

Phenotype of the knock-down of putative T. brucei Naa30 by RNAi. TcNaa30 protein expression analysis of wild type (W), transformant (T), noninduced (-), and induced (+) cells by western blotting. Cells were counted, washed, and dissolved in sample buffer and immediately boiled. About equal amount of each sample was used in the experiment. Note that a band of about 17 kDa, whose identity is not known, was also identified. GAPDH was used as loading control.