The Chronology of Angiostrongylus vasorum (Baillet, 1866), Kamensky, 1905: Infection in Biomphalaria glabrata (Say, 1818)

Figure 2

Histological sections of Biomphalaria glabrata infected with 1000 L1 of Angiostrongylus vasorum. Hematoxylin and eosin staining. (a) Larva in the foot fibromuscular tissue (arrows) presenting a clear space between it and the tissue with cellular infiltrate around five hours after the beginning of infection; (b) larvae in foot fibromuscular tissue with no evident cellular reaction with six hours after beginning of infection. White asterisk shows structures of the nervous system; (c) larva located in the columellar muscle with cellular infiltrate close to it; (d) larvae located in the lamellae of pseudobranquia (arrow), showing no cellular reaction, eight hours after the beginning of infection; (e) larvae in foot fibromuscular tissue (arrow) with evidence of perilarvar reaction more organized, maintaining a clear space of twelve hours after the beginning of infection; (f) intense cellular reaction in the fibromuscular tissue of dorsalis pedis mass with the presence of a pregranuloma perilarvar (white asterisk) twenty-four hours of the beginning of infection; (g) cell infiltration in the fibromuscular tissue of the dorsalis pedis region (black asterisk) with the starting to form a cell button on the one side of the pregranulomatous reaction (arrow); (h) larva located in the mantle without showing obvious cellular reaction maintaining the standard of formation of a clear space with forty-eight of the beginning of infection. Magnifications: (a, c, d, g, and h) 35 μm; (b) 50 μm; (e) 25 μm; (f) 40 μm.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)