Review Article
DNA-Detection Based Diagnostics for Taenia solium Cysticercosis in Porcine
Table 5
Comparison between established PCR and most common isothermal amplification methods.
| | PCR | LAMP | RPA |
| Sample processing prior to amplification | Nucleic acid extraction required | Amplification from crude samples possible | Amplification from crude samples possible [22] | Simplicity of operation | Complex | Comparatively easier to setup than PCR | Relatively easier to setup than PCR | Cost | $6–7.7 [70] | $0.71–2 | $4.3−4.7 [64, 69] | Stability of reagent | Cold chain required for enzymes | Cold chain required for enzymes | Reagents available as dry pellet [17] | | | Reagents available as dry pellet [52, 53] | | Skills required | High | Moderate | Moderate | Reaction time | 2 h [14] | 60−90 mins [13, 21] | 15−30 mins [22] | Amplification temperature | Varying [32, 33] | 65°C [43] | 25−42°C [17, 22] | Sensitivity | High | High | High | Specificity | High | High | High | Limit of detection | 1 ng [25, 70] | 5 eggs/g of feces [13] | Unknown | Taenia solium identification | Yes [14, 26, 27] | Yes [12, 13, 42] | Possible | Field tested | No | Yes | No | Product developer | Qiagen, Hilden, German [70] | New England Biolabs, Hitchin, UK | TwistDx, Cambridge, UK [20] |
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