Optimization of a Loop-Mediated Isothermal Amplification Assay as a Point-of-Care Tool for the Detection of Wuchereria bancrofti in Human Blood in Tana River Delta, Kenya

Figure 7

PCR sensitivity testing on gel electrophoresis. Lane ML: molecular marker 100 bp; lane PC: positive control, W. bancrofti DNA; lanes 10⁻¹–10⁻⁷: serial dilutions of DNA extracts for testing the assay sensitivity; lane 10⁻⁸: no amplification; lane NC: represents negative control; 10⁻¹–10⁻⁷ showed amplification of W. bancrofti as bands appeared just below the 200 bp as the expected band size was 188 bp. This showed that PCR assay was sensitive and could detect to up to 1/1000000 (1/10⁷) copies of parasite DNA in the diluent, while no band was observed in 1/10000000 (1/10⁸) copies in the diluent.