Optimization of a Loop-Mediated Isothermal Amplification Assay as a Point-of-Care Tool for the Detection of Wuchereria bancrofti in Human Blood in Tana River Delta, Kenya

Table 2

Time-dependent testing.

Sample	Incubation time (minutes)
	30-minute amplification	40-minute amplification	60-minute amplification
W. bancrofti-positive control	Green color	Green color	Green color
W. bancrofti-positive specimen (K19)	Light green color	Green color	Green color
S. mansoni	Orange color	Orange color	Orange color
Negative control	Orange color	Orange color	Orange color
Master Mix -Blank	Orange color	Orange color	Orange color

The light-green/green color indicated the presence of W. bancrofti DNA while the orange color indicates that no amplification took place.