Genetic Diversity of Merozoite Surface Protein-1 and -2 Genes in Plasmodium falciparum Isolates among Asymptomatic Population in Boset and Badewacho Districts, Southern Ethiopia

Table 1

Sequences of the primers for msp-1 and msp-2 genes in Plasmodium falciparum isolates.

Locus	PCR round	Primer	Primer sequences^a
MSP-1 (block 2)	Primary PCR	msp1-F1	5-CTA GAA GCT TTA GAA GAT GCA GTA TTG-3
		msp1-R1	5-CTT AAA TAG TAT TCT AAT TCA AGT GGA-3
		K1-F	5-AAT GAA GAA GAA ATT ACT CA AAA GGT-3
		K1-R	5-GCT TGC ATC AGC TGG AGG GCT TGC ACC-3
	Secondary PCR	MAD20-F	5-AAA TGA AGG AAC AAG TGG AAC AGC TGT-3
		MAD20-R	5-ATC TGA AGG ATT TGT ACG TCT TGA ATT-3
		R033-F	5-TAA AGG ATG GAG CAA ATA CTC AAG TTG-3
		R033-R	5-CAT CTG AAG GAT TTG CAG CAC CTG GAG-3
Msp-2 (block 3)	Primary PCR	msp2-F1	5-ATG AAG GCA ACT AAA ACA TTG TCT ATT-3
		msp2-R1	5-CTT TGT TAC CAT CGG TAC ATT CTT-3
		3D7-F	5-GCA GAA AGT AAG CCT TCT ACT GGT GCT-3
	Secondary PCR	3D7-R	5-GAT TTG TTT CGG CAT TAT TAT-GA-3
		FC27-F	5-GCA AAT GAA GGT TCT AAT ACT AAT AG-3
		FC27-R	5-GCT TTG GGT CCT TCT TCA GTT GAT TC-3

^aAs described previously by Khaireh et al. [12] and Hamid et al. [14].