Genetic Diversity of Merozoite Surface Protein-1 and -2 Genes in Plasmodium falciparum Isolates among Asymptomatic Population in Boset and Badewacho Districts, Southern Ethiopia

Table 4

Distribution of Plasmodium falciparum msp-1 and msp-2 allelic family profile by age groups, sex, and study site.

Gene ()	Type of alleles	Age group (years), n (%)					Sex, n (%)		Study site, n (%)
		<5	5–14	15–24	25–34	>34	Male	Female	Badewacho	Boset
msp-1	MAD20	1 (10)	1 (10)	2 (20)	4 (40)	2 (20)	6 (60)	4 (40)	9 (90)	1 (10)
	K1	0 (0)	0 (0)	1 (20)	3 (60)	1 (20)	3 (60)	2 (40)	4 (80)	1 (20)
	RO33	0 (0)	2 (25)	1 (12.5)	3 (37.3)	2 (25)	4 (50)	4 (50)	2 (25)	6 (75)
	MAD20 + K1	2 (16.7)	2 (16.7)	3 (25)	1 (8.3)	4 (33.3)	6 (50)	6 (50)	12 (100)	0 (0)
	MAD20 + RO33	0 (0)	2 (40)	1 (20)	2 (40)	0 (0)	3 (60)	2 (40)	2 (40)	3 (60)
	K1 + RO33	0 (0)	0 (0)	0 (0)	1 (50)	1 (50)	2 (100)	0 (0)	2 (100)	0 (0)
	MAD20 + K1 + RO33	0 (0)	0 (0)	0 (0)	0 (0)	1 (100)	0 (0)	1 (100)	1 (100)	0 (0)
	Chi-square, χ^2			16.385			3.263			19.89
	-value			0.875			0.775			0.003
	MOI for msp-1	1.7	1.6	1.5	1.4	1.8	1.5	1.6	1.5	1.4
	-value			0.268			0.970			0.436
msp-2	FC27	1 (25)	0 (0)	0 (0)	2 (50)	1 (25)	2 (50)	2 (50)	4 (100)	0 (0)
	3D7	0 (0)	5 (31.3)	4 (25)	4 (25)	3 (18.8)	10 (62.5)	6 (37.5)	9 (56.3)	7 (43.8)
	FC27+ 3D7	2 (8.7)	2 (8.7)	4 (17.4)	8 (34.8)	7 (30.4)	12 (52.2)	11 (47.8)	19 (82.6)	4 (17.4)
	Chi-square			9.05			0.468			4.96
	P-value			0.338			0.791			0.084
	MOI for msp-2	1.3	1.3	1.5	1.6	1.8	1.5	1.7	1.6	1.4
	-value			0.607			0.325			0.385