Prevalence, Phytochemical Investigation, and In Vitro Acaricidal Efficacy Evaluation of Dodonaea angustifolia, Eucalyptus globulus, Millettia ferruginea, and Euphorbia abyssinica against Sarcoptic Mange of Camel, Babile District, Ethiopia

Table 1

(a) The overall Prevalence of mange mites based on different risk factors

Risk factors	Categories	No. examined animal	No. positive animal	Prevalence		value
Kebeles	Daketa	128	15	11.7	2.612^a	0.271
	Anod	128	24	18.8
	Mullu	128	22	17.2
	Total	384	61	15.9
Sex	Female	131	21	16.1	0.003^a	0.532
	Male	253	40	15.8
	Total	384	61	15.9
Age	Adult	274	30	10.95	17.445^a	0.001
	Young	110	31	28.2
	Total	384	61	15.9
Body condition	Poor	129	34	26.4	29.90^a	0.001
	Medium	163	22	13.5
	Good	92	5	5.4
	Total	384	61	15.9

(b) Percentage yield of crude/oil extracts of the selected medicinal plants leaf

Plants	Plant part extracted	Solvents for extraction	Weight of dry powder (g)	Weight of dry extract (g)	Yield %	Means of dilution
D. angustifolia	Leaf	Methanol	500	73	14.6	2% Tween 80
M. ferruginea	Leaf	Methanol	500	97	19.4	2% Tween 80
E. globulus	Leaf	Distilled water	500	38	7.6	2% Tween 80
E. abyssinica	Leaf	Methanol	400	69	17.25	2% Tween 80

(c) Qualitative determinations of active ingredients in crude/oil extracts of the selected plants

Secondary metabolites	D. angustifolia	M. ferruginea	E. globulus	E. abyssinica
Saponin	+	—	+	+
Tannin	—	+	+	+
Phenol	+	+	+	+
Steroids	+	+	+	—
Flavonoids	+	+	+	+
Phlobatannin	+	+	+	—
Glycosides	+	+	+	—
Triterpenes	+	+	+	—
Alkaloids	+	+	—	—

Note: +: present, -: absent (negative).

(d) Comparisons on acaricidal activity of different extracts on mange mites after 10-min exposure

Dose (mg/mL)	Mean number of mite dead () post exposure
	D. angustifolia	M. ferruginea	E. globulus	E. abyssinica
200
100
50
25
12.5
6.25
2% Tween 80
1% diazinon

(e) Comparisons on acaricidal activity of different extracts on mange mites after 20-min exposure

Dose (mg/mL)	Mean number of mite dead () postexposure
	D. angustifolia	M. ferruginea	E. globulus	E. abyssinica
200
100
50
25
12.5
6.25
2% Tween 80
1% diazinon

(f) Comparisons on acaricidal activity of different extracts on mange mites after 40-min exposure

Dose (mg/mL)	Mean number of mite dead () postexposure
	D. angustifolia	M. ferruginea	E. globulus	E. abyssinica
200
100
50
25
12.5
6.25
2% Tween 80
1% diazinon

(g) Comparisons on acaricidal activity of different extracts on mange mites after 80-min exposure

Dose (mg/mL)	Mean number of mite dead () postexposure
	D. angustifolia	M. ferruginea	E. globulus	E. abyssinica
200
100
50
25
12.5
6.25
2% Tween 80
1% diazinon

(h) Comparisons on acaricidal activity of different extracts on mange mites after 180-min exposure

Dose (mg/mL)	Mean number of mite dead () postexposure
	D. angustifolia	M. ferruginea	E. globulus	E. abyssinica
200
100
50
25
12.5
6.25
2% Tween 80
1% diazinon