Angiotensin II Induces Differentiation of Human Neuroblastoma Cells by Increasing MAP2 and ROS Levels

Figure 1

Morphological changes in neuroblastoma cells after Ang II treatment. Cells were incubated with Ang II (600 nM) for 24 h, and cell size changes were visualized by bright-field microscopy. Changes in cell size were also analyzed by flow cytometry. (a) Untreated neuroblastoma cells visualized in bright-field. (b) Ang II-treated neuroblastoma cells visualized in bright field. Changes in cell size and shape (b) were observed in cells exposed to Ang II when compared to untreated (a). (c) Representative flow cytometric histogram of untreated (dark peak) and Ang II-treated (gray peak) neuroblastoma cells. (d) Cell size significantly decreased after incubation with Ang II (dark bar) when compared to untreated (open bar). Each bar represents the average of five experiments. Vertical lines at each bar represent SEM (). FSC: forward scatter.