Angiotensin II Induces Differentiation of Human Neuroblastoma Cells by Increasing MAP2 and ROS Levels

Figure 3

Effect of Ang II in the generation of intracellular ROS and cell viability. Human neuroblastoma cells were cultured in the presence of Ang II (600 nM) for 24 h, and intracellular ROS levels and the percentage of healthy, apoptotic, or necrotic cells were analyzed by flow cytometry. (a) Ang II increased significantly the intracellular ROS levels in neuroblastoma cells when compared to untreated. (b) No significant differences were observed in the percentage of healthy, apoptotic, or necrotic cells in Ang II-treated neuroblastoma cells when compared to untreated. Each bar represents the average of three experiments. Vertical lines at each bar represent SEM (). MFI: mean fluorescence intensity.