Angiotensin II Induces Differentiation of Human Neuroblastoma Cells by Increasing MAP2 and ROS Levels

Figure 4

Effect of apocynin on neuroblastoma MAP2 expression levels and ROS production induced by Ang II. Cells were incubated with Ang II (600 nM) alone or Ang II plus apocynin (500 μM) for 24 h, and the levels of MAP2 and intracellular ROS were quantified by flow cytometry. A significant increase was observed on MAP2 (a) and intracellular ROS levels (b) when the cells were incubated with Ang II alone (dark gray bar) compared to untreated (open bar). Both MAP2 and intracellular ROS levels decreased significantly when the cells were exposed to Ang II plus apocynin (dark bar) compared to those exposed to Ang II alone (dark gray bar). No significant differences were observed in the levels of MAP2 (a) or intracellular ROS (b) between cells that were exposed to Ang II plus apocynin (dark bar) and untreated (open bar) or apocynin alone (light gray bar). Each bar represents the average of four experiments. Vertical lines at each bar represent SEM (; ). MFI: mean fluorescence intensity.