Angiotensin II Induces Differentiation of Human Neuroblastoma Cells by Increasing MAP2 and ROS Levels

Figure 5

Effect of selective AT1 or AT2 receptor blockers on neuroblastoma MAP2 expression levels induced by Ang II. Cells were incubated with Ang II (600 nM) alone, Ang II plus Losartan (10^-7 M), or Ang II plus PD123.319 (10^-7 M) for 24 h, and the levels of MAP2 were quantified by flow cytometry. A significant increase was observed in MAP2 levels when the cells were incubated with Ang II alone compared to untreated. The levels of MAP2 decreased significantly when the cells were exposed to Ang II plus PD123.319 compared to those exposed to Ang II alone. However, no significant differences were observed when the cells were incubated with Ang II plus Losartan compared to those exposed to Ang II alone. Each bar represents the average of four experiments. Vertical lines at each bar represent SEM (). MFI: mean fluorescence intensity.