Angiotensin II Induces Differentiation of Human Neuroblastoma Cells by Increasing MAP2 and ROS Levels

Figure 6

Effect of Ang II metabolism inhibitors on neuroblastoma MAP2 expression levels. Cells were incubated with Ang II (600 nM) alone, Ang II plus AP-N inhibitor (10^-7 M), or Ang II plus MLN-4760 (10^-7 M) for 24 h, and the levels of MAP2 were quantified by flow cytometry. A significant increase was observed on MAP2 levels when the cells were incubated with Ang II alone compared to untreated. However, no significant differences were observed in the levels of MAP2 when the cells were exposed to Ang II alone or Ang II plus AP-N inhibitor or MLN-4760. Each bar represents the average of four experiments. Vertical lines at each bar represent SEM (). MFI: mean fluorescence intensity.