Berberine Reshapes the Balance of the Local Renin-Angiotensin System by Modulating Autophagy under Metabolic Stress in Pancreatic Islets

Figure 5

Knockout of ACE2-induced autophagy formation in vivo. (a) Representative images of immunofluorescence staining for insulin (green) and ACE2 (red) (×400). WT (): wild type C57BL/6 J mice; ob/ob (): ob/ob mice; Ob-BBR (): berberine-treated ob/ob mice. (b) Representative images of the islet cell structure by TEM. The arrows show autophagosomes. (c, d) Representative images of the islet cell structure by immunohistochemistry (×400). WT (): wild type C57BL/6 J mice; ACE2 KO (): ACE2 KO mice.