Aldosterone Induces the Proliferation of Renal Tubular Epithelial Cells In Vivo but Not In Vitro

Figure 2

Eplerenone inhibits the expression of PCNA in aldosterone-fusion mice. Immunohistochemical staining for PCNA in kidney sections from different groups. (a) Sham group. (b) UN group: the left kidney of the mouse was removed, and the right kidney was collected 6 weeks later. (c) UA group: aldosterone (0.75 μg·h⁻¹) was administered for 6 weeks. (d) UAE group: eplerenone was given to the UA group via diet at 1.25 g·kg⁻¹ diet (equal to 100 mg·kg⁻¹d⁻¹) for 6 weeks, and other groups of mice were fed regular chow. (e) Quantification of PCNA. Data are the (). vs. sham group, vs. sham group, vs. sham group; ^# vs. UA group, ^## vs. UA group, ^### vs. UA group. Original magnification, ×400. PCNA: proliferating cell nuclear antigen; UA group: unilateral nephrectomy + aldosterone perfusion; UN group: unilateral nephrectomy; UAE group: UA + eplerenone.

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