Research Article
Aldosterone Induces the Proliferation of Renal Tubular Epithelial Cells In Vivo but Not In Vitro
Figure 3
Effects of aldosterone on the protein expression of PCNA according to western blotting. (a) HK2 cells were treated with aldosterone (0, 10−9, 10−8, 10−7, and 10−6 mol/L) for 3, 6, 12, 24, 36, or 48 h. Total cellular protein was extracted, and 30 μg of protein was loaded for western blotting of total PCNA and β-actin. (b) mDCT cells. Data are the (). HK2: human kidney proximal tubular epithelial; mDCT: mouse distal convoluted tubule epithelial; PCNA: proliferating cell nuclear antigen.
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