Research Article
Aldosterone Induces the Proliferation of Renal Tubular Epithelial Cells In Vivo but Not In Vitro
Figure 7
Growth of HK2 cells and mDCT cells observed by live imaging. Cells ( cells/mL) were plated into 96-well plates and stimulated by aldosterone (0, 10−9, 10−8, 10−7, and 10−6 mol/L) for 48 h. The time interval for recording images was 1 h. After 12 h, the cells began to adhere to the wall. We observed cell growth for 3, 6, 12, 24, 36, and 48 h. (a) HK2 cells. (b) mDCT cells. (A) Aldosterone concentration of 0 mol/L. (B) Aldosterone concentration of 10−9 mol/L. (C) Aldosterone concentration of 10−8 mol/L. (D) Aldosterone concentration of 10−7 mol/L. (E) Aldosterone concentration of 10−6 mol/L. 1, 2, 3, 4, 5, and 6 show cell growth at 3, 6, 12, 24, 36, and 48 h, respectively. We selected three replicate wells randomly, and the number of cells and the degree of fusion were calculated. Results are plotted as a bar chart, and values are the . (A1, B1) represent changes in the cell number, and (A2, B2) represent changes in cell fusion, compared with that in the group not given aldosterone.
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