Research Article

A New FRET-Based Sensitive DNA Sensor for Medical Diagnostics using PNA Probe and Water-Soluble Blue Light Emitting Polymer

Figure 3

Variation of PL emission intensity of acceptor, PNAC* (at excitation wavelength 450 nm) for different Acceptor/Donor ratios (A:D) in the range from 1:0 to 1:2 in the solution of PNAC* and complementary ssDNA. At 1:1 ratio, the saturation of FRET is observed, there is no further increase in PL emission for higher A: D ratios.
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