A New FRET-Based Sensitive DNA Sensor for Medical Diagnostics using PNA Probe and Water-Soluble Blue Light Emitting Polymer
Figure 3
Variation
of PL emission intensity of acceptor, PNAC* (at excitation wavelength
450 nm) for different Acceptor/Donor ratios (A:D) in the range from 1:0 to
1:2 in the solution of PNAC* and complementary ssDNA. At 1:1 ratio, the saturation
of FRET is observed, there is no further increase in PL emission for
higher A: D ratios.