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Journal of Sensors
Volume 2008, Article ID 346016, 11 pages
http://dx.doi.org/10.1155/2008/346016
Research Article

Near-Infrared Resonance Energy Transfer Glucose Biosensors in Hybrid Microcapsule Carriers

1Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA 71272, USA
2Biomedical Engineering Program, Louisiana Tech University, Ruston, LA 71272, USA
3Department of Biomedical Engineering, Texas A&M University, 337 Zachry Engineering Center, College Station, TX 77843-3120, USA

Received 10 June 2008; Accepted 20 July 2008

Academic Editor: Igor Medintz

Copyright © 2008 Swetha Chinnayelka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Fluorescence-based sensing systems offer potential for noninvasive monitoring with implantable devices, but require carrier technologies that provide suitable immobilization, accessibility, and biocompatibility. Recent developments towards this goal include a competitive binding assay for glucose that has been encapsulated in semipermeable microcapsule carriers. This paper describes an extension of this work to increase the applicability to in vivo monitoring, wherein two significant developments are described: (1) a near-infrared resonance energy transfer system for transducing glucose concentration, and (2) novel hybrid organic-inorganic crosslinked microcapsules as carriers. The quenching-based assay is a competitive binding (CB) system based on apo-glucose oxidase (AG) as the receptor and dextran as the competitive ligand. The encapsulated quencher-labeled dextran and near infrared donor-labeled glucose receptor showed a stable and reversible response with tunable sensitivity of 1–5%/mM over the physiological range, making these transducers attractive for continuous monitoring for biomedical applications.