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Journal of Sensors
Volume 2009, Article ID 671896, 6 pages
Research Article

A Novel Biosensor to Detect MicroRNAs Rapidly

1National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
2Center for Infection and Immunity, Institute of Biophysics, Graduate School of the Chinese Academy of Sciences (CAS), Beijing 100080, China

Received 6 January 2009; Revised 13 May 2009; Accepted 9 July 2009

Academic Editor: Wojtek Wlodarski

Copyright © 2009 Jie-Ying Liao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


-free -ATPase within chromatophore was constructed as a novel biosensor to detect miRNA targets. Specific miRNA probes were linked to each rotary subunits of -ATPase. Detection of miRNAs was based on the proton flux change induced by light-driven rotation of -free -ATPase. The hybridization reaction was indicated by changes in the fluorescent intensity of pH-sensitive CdTe quantum dots. Our results showed that the assay was attomole sensitivities (  mol) to target miRNAs and capable of distinguishing among miRNA family members. Moreover, the method could be used to monitor real-time hybridization without any complicated fabrication before hybridization. Thus, the rotary biosensor is not only sensitive and specific to detect miRNA target but also easy to perform. The -free -ATPase-based rotary biosensor may be a promising tool for the basic research and clinical application of miRNAs.