Quantitative, Label-Free Detection of the Aggregation of α-Synuclein Using Microcantilever Arrays Operated in a Liquid Environment

Figure 1

Schematic showing the functionalisation of the cantilever array. The backside of the cantilever is coated with a PEG silane monolayer to prevent nonspecific adsorption of α-synuclein during the experiment. The array is coated with a Ti/Au layer to facilitate functionalisation using thiol chemistry. The reference cantilever is coated with a self-assembled OH monolayer. The test cantilever is coated with α-synuclein bound to a DSU monolayer. All remaining binding sites are blocked with BSA.