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Journal of Sensors
Volume 2015, Article ID 293461, 8 pages
http://dx.doi.org/10.1155/2015/293461
Research Article

Efficient and Rapid Detection of Salmonella Using Microfluidic Impedance Based Sensing

1Department of Electrical and Computer Engineering, University of Missouri, Columbia, MO 65211, USA
2Cooperative Research and Life & Physical Sciences, Lincoln University, Jefferson City, MO 65101, USA

Received 16 November 2014; Revised 18 January 2015; Accepted 29 January 2015

Academic Editor: Stephane Evoy

Copyright © 2015 Shibajyoti Ghosh Dastider et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We present a low cost, easy to fabricate biosensor, which can quickly and accurately detect Salmonella typhimurium. This study also compares the advantages of the microfluidic biosensor over a nonmicrofluidic biosensor. High density interdigitated electrode array was used to detect Salmonella cells inside a microfluidic chip. Monoclonal anti-Salmonella antibodies were allowed to be immobilized on the surface of the electrode array for selective detection of Salmonella typhimurium. An impedance analyzer was used to measure and record the response signal from the biosensor. The biosensor provides qualitative and quantitative results in 3 hours without any enrichment steps. The microfluidic biosensor’s lower detection limit was found to be  CFU/mL compared to the  CFU/mL of the nonmicrofluidic biosensor, which shows that the microfluidic biosensor has 10-fold increased sensitivity. The impedance response of microfluidic biosensor was also significantly higher (2 to 2.9 times) compared to the nonmicrofluidic biosensor.