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Journal of Sensors
Volume 2015 (2015), Article ID 768352, 6 pages
Research Article

Noninvasive Superoxide Monitoring of In Vitro Neuronal Differentiation Using a Cell-Based Biosensor

Faculty of Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece

Received 27 April 2015; Accepted 25 June 2015

Academic Editor: Suna Timur

Copyright © 2015 Georgia Moschopoulou and Spyridon Kintzios. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Membrane-engineered cells bearing superoxide dismutase (SOD) molecules on their surface offer the capability of ultrarapid and ultrasensitive detection of the superoxide anion () through the measurement of changes of their cell membrane potential. We herewith report the application of this technology for the noninvasive determination of superoxide levels during the in vitro differentiation of PC12 cells. We were able to detect changes in accumulation in the culture medium, which were closely associated with the progress of neuronal differentiation.