Feasibility Study on a Measurement Method and a Portable Measuring System to Estimate the Concentration of Cloxacillin and β-Lactamase in Milk

Table 2

Performed feasibility tests and relative component concentrations. Buffer (, pH 7.00) volume added to reach the final cuvette volume of 300 l. Milk volume added to reach the final cuvette volume of 300 l.

Component	Stock	Cuvette
	concentration	concentration
FT1, buffer
Buffer
CENTA	5 mM	240 M
AmpC	13.85 M	130 nM
FT2, milk
Milk
CENTA	5 mM	240 M
AmpC	13.85 M	130 nM
FT3, antibiotic
Milk
CENTA	5 mM	240 M
AmpC	13.85 M	130 nM
Cloxacillin	0.5 M	5 nM
		10 nM