Comparative Assessment of Affinity-Based Techniques for Oriented Antibody Immobilization towards Immunosensor Performance Optimization

Figure 2

Normalized fluorescence intensities from (A) the immobilized Cy5-labeled secondary antibodies in CBB pH 9.2 for 1 hour and upon incubation with (B) 10 mg/ml glucose in PBS 1× pH 7.4 for 1 hour or (C) 10 mg/ml glucose in CBB pH 9.2 for 1 hour.