Inhibition of Epidermal Growth Factor Receptor and PI3K/Akt Signaling Suppresses Cell Proliferation and Survival through Regulation of Stat3 Activation in Human Cutaneous Squamous Cell Carcinoma

Table 1

Immunohistochemical analysis of human cutaneous SCC tissues.

Case	Age	Sex	Site	p-EGFR	p-Akt	p-Stat3	EMSA Stat3
1	64	M	Back, primary lesion	+	−	+	N.D.
1′			Lymph node, metastasis of case 1	+	−	+	N.D.
2	89	F	Face	+	+	+	N.D.
3	41	F	Foot	−	+	−	N.D.
4	65	M	Head	+	+	+	N.D.
5	61	F	Hand, primary lesion	+	+	+	+
5′			Lymph node, metastasis of case 5	+	+	+	+
6	58	M	Thigh	−	+	−	−
7	81	M	Foot finger	+	+	+	+
8	54	F	Lower leg	−	−	−	N.D.
9	41	F	Hand finger	+	−	+	N.D.
10	72	M	Forehead	+	+	+	+
11	81	F	Face	+	−	+	N.D.
12	72	F	Face	+	+	+	N.D.
13	75	M	Face	+	+	+	N.D.
14	90	F	Face	−	−	−	N.D.
15	62	M	Face	+	+	+	N.D.
16	64	M	Face	+	+	+	N.D.
17	80	F	Face	−	−	+	N.D.
18	96	F	Face	+	+	+	N.D.
19	88	M	Head	−	+	+	N.D.
20	81	M	Lower leg	+	+	+	N.D.
21	77	M	Head	+	+	+	N.D.
22	89	F	Head	+	−	+	N.D.
23	71	F	Face	−	+	+	N.D.
24	93	F	Vulva	+	+	+	N.D.
25	71	M	Foot back	+	+	+	N.D.
26	81	M	Head	+	−	+	N.D.
27	55	F	Sole	−	−	+	N.D.
28	101	M	Face	+	−	+	N.D.
29	87	F	Forearm	+	−	+	N.D.
29′			Lymph node, metastasis of case 29	+	+	+	N.D.
30	73	M	Thigh	−	−	+	N.D.
31	87	M	Scrotum	+	−	+	N.D.
32	74	F	Lip	−	−	−	N.D.
			Positive cases/Total	25/35	20/35	30/35	4/5

+: positive, −: negative, N.D.: not done.