Protein Kinase Cε, Which Is Linked to Ultraviolet Radiation-Induced Development of Squamous Cell Carcinomas, Stimulates Rapid Turnover of Adult Hair Follicle Stem Cells
Figure 4
Detection of label retaining cells (LRC) by immunostaining using antibody to BrdU: to identify the LRCs, newborn mice (3 days old) were injected subcutaneously with BrdU (50 mg/kg body weight) twice daily for 3 days. Mice were sacrificed at 3, 6, and 8 weeks after BrdU injection. Shown are the representative photographs of BrdU-labeled cells from paraffin-fixed skin sections from WT and TG mice. The white arrow points to BrdU positive cells in the bulge region of hair follicle. In all the figures, the asterisk is the autofluorescence of the hair shaft. The incorporation and retention of BrdU are shown in the various parts of hair follicle at 3, 6, and 8 weeks in the indicated mice (a)–(h). (i)–(k) are showing the dual labeling of BrdU and k15 expressing cells in the bulge region of hair follicle.
