Kaposi’s Sarcoma-Associated Herpesvirus Subversion of the Anti-Inflammatory Response in Human Skin Cells Reveals Correlates of Latency and Disease Pathogenesis
Figure 2
Differential induction of viral lytic cycle in skin cells reveals cell line-specific correlates of latency. (a) and (b): Total DNA was extracted from rKSHV.219-infected MeWo-KSHV cells (a) or Mel1700-KSHV cells (b) either mock-treated or treated with increasing concentrations of NaB, as indicated. An equal amount of normalized viral DNA was then used for PCR amplification of the virus genome using ORF26-specific primers (Table S1). Approximately 10 ng of DNA from KSHV-infected BCBL-1 cells was used as a positive control. (c) and (d): Representative phase, GFP, RFP, and color-merged images of infected MeWo-KSHV (c) or Mel1700-KSHV cells (d) either untreated or treated with 2 mM NaB for two days.