Kaposi’s Sarcoma-Associated Herpesvirus Subversion of the Anti-Inflammatory Response in Human Skin Cells Reveals Correlates of Latency and Disease Pathogenesis

Figure 3

rKSHV.219-infected melanoma cells support the full spectrum of latent and lytic viral gene expression. Total RNA from mock (−) or rKSHV.219-infected (+) MeWo and Mel1700 cells either left untreated (−) or induced (+) with 2 mM NaB for two days was used as template for RT-PCR amplification of viral transcripts using primer sets specific for genes belonging to the latent class I (LANA, vFLIP, vCyclin), latent/inducible class II (RTA, vGPCR, vIL6), or the strictly lytic class III (gB, gH, K8.1) (Table S1). GAPDH was used as a template loading control.