Kaposi’s Sarcoma-Associated Herpesvirus Subversion of the Anti-Inflammatory Response in Human Skin Cells Reveals Correlates of Latency and Disease Pathogenesis
Figure 8
KSHV impairment of the melanogenic response is a virologic correlate of latency in skin cells. Uninfected (−) and rKSHV.219-infected (+) MeWo cells (a) or Mel1700-cells (b) were plated on chamber slides without (control) or with α-MSH and allowed to adhere overnight. Cells were then fixed, permeabilized, and stained with anti-TRP-1 antibody and counterstained with DAPI nuclear stain. Shown are representative 63x magnification (oil immersion) images of representative cells from each field of view. White arrows depict ER/Golgi-associated TRP-1 (apparently present in untreated MeWo and in α-MSH-treated MeWo-KSHV cells), whereas green arrows point to outgrowths of promelanogenic dendritic spines induced by α-MSH in MeWos ((a) bottom left) and in KSHV-infected Mel1700, in which they appear to increase even more profoundly upon treatment with α-MSH (b). For wide-view 20x images of the same treatments, please see Figure S11.
