Microvessel Density in Patients with Cutaneous Melanoma: An Up-to-Date Systematic Review and Meta-Analysis

Table 2

MVD assessment.

Author	MVD assessment method	Antibody	Magnification used	Spots examined	Blinded reading	Observers	Separate count for intra/peritumoral vessels	MVD cut-off
Döme et al.	Immunohistochemistry, light microscopy, transmission electron microscopy	CD-31, anti-laminin	20x, 40x	2	n/a	n/a	Yes	30 microvessels/mm^2
Hillen et al.	Light microscopy, immunohistochemistry	CD-31, CD-34	200x	4	Yes	2	Yes	n/a
Ilmonen et al.	Light microscopy, immunohistochemistry	CD-31	n/a	n/a	n/a	n/a	No	20 microvessels/mm^2
Massi et al.	Light microscopy, immunohistochemistry	CD-31	10x, 40x	n/a	n/a	n/a	No	Microvessel area: 389.3770/μm2
Pastushenko et al.	Light microscopy, immunohistochemistry, Chalkley score	CD-34	100x, 200x, 400x	3	n/a	n/a	Yes	1.67 microvessels/mm^2
Ribatti et al.	Light microscopy, immunohistochemistry	VIII factor	250x	4–6	Yes	2	No	10 microvessels/mm^2
Shimizu et al.	Light microscopy, immunohistochemistry	CD-34	400x	4	Yes	>2	No	4 microvessels/mm^2
Straume et al.	Light microscopy, immunohistochemistry	VIII factor, CD105	25x, 100x, 400x	<10	n/a	n/a	Yes	94 microvessels/mm^2
Valencak et al.	Light microscopy, immunohistochemistry	CD-31	40x, 200x	3	Yes	2	No	32.33 microvessels/field