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Volume 12, Issue 1, Pages 1-8

Triterpene glycosides from transformed root cultures of Astragalus mongholicus BGE

R. Tappe,1 H. Budzikiewicz,1 I. Ionkova,2 and A. W. Alfermann3

1Institut für Organische Chemie der Universität zu Köln, Greinstr. 4, D-50939 Koln, Germany
2Institute for Pharmacology and Pharmacy Medical Academy, Dtmaw 2, BG-l000 Sofia, Bulgaria
3Institut für Entwickltmgs- und Molekularbiologie der Pflanzen der Universität DUsseldorf, Universitätsstr. 1, D-40225 Düsseldorf, Germany

Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Transfonned root cultures of Astragalus mongholicus Bge. have been established by infecting of sterile grown plants with AgrobacterilDfl rhizogenes strains LBA 9402 and ATCC 15834. A promoting effect of acetyl syringone on the Agrobacteriwn-mediated hairy root (HR) initiation was obselVed. The transfonned root clones were assayed for opines by electrophoresis and 1LC. All of them were positive for agropine/mannopine. It was shown, that Astragalus-hairy roots (HR) of the transfonned cultures produce the same kind of saponins in considerable amounts as those of intact plants. The triterpene saponins have been isolated from the methanol extract of the HR and identified as cycloartane derived saponins. On the basis of analytical data obtained by mass spectrometry (NI-F AB-MS, EI-MS), and homo- and heteronuclear NMR experiments (DEPT, DQF-COSY, HOHAHA, ROESY, HMQC, HMBC), the structures of three triterpenoid glycosides were determined as astragaloside I, IT, ill [1,2]. A complete assignment of the IH_ and 13C-NMR data is given.