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Spectroscopy
Volume 12, Issue 2, Pages 43-53
http://dx.doi.org/10.1155/1994/982952

Electrospray Tandem Mass Spectrometry of Ecdysteroid Moulting Hormones

Jocelyne Hellou, Joseph Banoub, Emmanuel Gentil, Dave M. Taylor, and Paul G. O’Keefe

Department of Fisheries & Oceans, Northwest Atlantic Fisheries Center, Science Branch, Toxicology Section P.O. Box 5667, St John’s Newfoundland, A1C 5X1, Canada

Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Electrospray mass spectrometIy has aided the structural characterization of ecdysteroid moulting hormones as a means of understanding factors controlling the moulting cycle in crabs. Possible fragmentation routes were frrst obtained by controlled collision-activated dissociation (CAD) initiated by cone voltage fragmentation. Low energy CAD MSIMS analyses of the protonated molecules [M+H]+ confrrmed the characteristic fmgerprint patterns obtained by cone voltage fragmentation. MSIMS analyses of selected intermediate fragments provided additional structural data and established the fragmentation routes.