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Spectroscopy
Volume 16 (2002), Issue 3-4, Pages 207-216
http://dx.doi.org/10.1155/2002/326454

Magnetization‒transfer 31P NMR of biochemical exchange in vivo: Application to creatine kinase kinetics

Harald E. Möller1 and Dirk Wiedermann2

1Max‒Planck‒Institut für neuropsychologische Forschung, Stephanstraße 1a, D‒04103 Leipzig, Germany
2Max‒Planck‒Institut für neurologische Forschung, Gleueler Straße 50, D‒50866 Köln, Germany

Copyright © 2002 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Phosphorus‒31 saturation‒transfer NMR spectroscopy provides an elegant means to study fluxes through the creatine kinase reaction in human skeletal muscle. To obtain reliable quantitative kinetic information, experimental imperfections, such as incomplete saturation and radiofrequency bleed over need to be addressed appropriately. In resting muscle, creatine kinase was near equilibrium both in normal controls and in a patient with impaired oxidative phosphorylation. Oral intake of high doses of creatine monohydrate for several days resulted in significantly increased concentrations of phosphocreatine but had no measurable effect on the phosphocreatine resynthesis rate in resting muscle.