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Spectroscopy
Volume 16, Issue 3-4, Pages 343-350
http://dx.doi.org/10.1155/2002/714548

Use of Synchrotron Radiation SAXS to Study the First Steps of the Interaction between Sodium Dodecyl Sulfate and Charged Liposomes

O. López,1 M. Cócera,1 R. Pons,1 H. Amenitsch,2 J. Caelles,1 J. L. Parra,1 L. Coderch,1 and A. de la Maza1

1Departamento de Tecnología de Tensioactivos, Instituto de Investigaciones Químicas y Ambientales de Barcelona (I.I.Q.A.B.), Consejo Superior de Investigaciones Científicas (C.S.I.C.), Calle Jorge Girona 18–26, 08034 Barcelona, Spain
2IBR, Austrian Academy of Sciences, ELETTRA, Sincrotrone Trieste, S.S. 14 Km 163.5, Bassovizza, 34012 Trieste, Italy

Copyright © 2002 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The technique of time resolved small angle X–ray scattering (SAXS) using a synchrotron radiation source was used to study the structural transformations as well as the kinetic associated with the first steps of the solubilization of liposomes induced by the anionic surfactant sodium dodecyl sulfate (SDS). Neutral and electrically charged (anionic and cationic) liposomes were used to investigate the effect of the electrostatic charges on these initial steps. The mechanism that induces the solubilization process consisted in an adsorption of surfactant on the bilayers and a desorption of mixed micelles from the liposomes surface to the aqueous medium. Regardless of the type of charge of the liposome the time needed for the desorption of the first mixed micelles was shorter than that for the complete adsorption of the surfactant on the liposomes surface. The present work demonstrates that the adsorption of the SDS molecules on liposomes was slower when the charges of surfactant and lipids were the same. As for the release of mixed micelles from the surface of these liposomes, this process was slower when the charges of surfactant and lipids were opposite.