Journal of Spectroscopy

Journal of Spectroscopy / 2003 / Article

Open Access

Volume 17 |Article ID 172838 | https://doi.org/10.1155/2003/172838

Jessica E. Prenni, Zhouxin Shen, Sunia Trauger, Wei Chen, Gary Siuzdak, "Protein characterization using Liquid Chromatography Desorption Ionization on Silicon Mass Spectrometry (LC-DIOS-MS)", Journal of Spectroscopy, vol. 17, Article ID 172838, 6 pages, 2003. https://doi.org/10.1155/2003/172838

Protein characterization using Liquid Chromatography Desorption Ionization on Silicon Mass Spectrometry (LC-DIOS-MS)

Abstract

This paper presents a novel combination of of-line liquid chromatography (LC) separation with desorption ionization on silicon mass spectrometry (DIOS-MS) as an alternative to the traditional on-line coupling of LC and electrospray ionization (ESI). In this work, electrospray deposition is used to generate a spatially preserved linear track of the separated sample on a specially designed DIOS chip. The total sequence coverage analysis of two model protein systems was evaluated by both LC-DIOS-MS and nanoLC ESI tandem MS (MS/MS). LC-DIOS-MS yielded improved sequence coverage for both of the model systems (between 99.5 and 100%) compared with traditional LC-ESI-MS/MS analysis (between 82 and 87.6%). In addition to improved sequence coverage determination, LC-DIOS-MS also offers the potential for high-throughput protein characterization.

Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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