Journal of Spectroscopy

Journal of Spectroscopy / 2003 / Article

Open Access

Volume 17 |Article ID 252581 | https://doi.org/10.1155/2003/252581

Kiyoto Kamagata, Kunihiro Kuwajima, "Parallel folding pathway of proline-free staphylococcal nuclease studied by the stopped-flow double-jump method", Journal of Spectroscopy, vol. 17, Article ID 252581, 10 pages, 2003. https://doi.org/10.1155/2003/252581

Parallel folding pathway of proline-free staphylococcal nuclease studied by the stopped-flow double-jump method

Abstract

The folding mechanism of proline‒free staphylococcal nuclease (SNase (pro)) (P11A, P31A, P42A, P47T, P56A, P117G) was investigated using the double‒jump stopped‒flow method (interrupted refolding). This method has enabled us to specifically monitor the amount of the native molecules during the refolding. The results indicate that the middle and slow phases observed in the refolding kinetics represent the formation of the native state (IM→N, IS→N) and that the folding mechanism of SNase (pro) is not represented by a single sequential pathway, but at least two parallel pathways are required for interpreting the results.

Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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