Journal of Spectroscopy

Journal of Spectroscopy / 2004 / Article
Special Issue

Second International Conference on Biomedical Spectroscopy: From the Bench to the Clinic, London, UK, 5–8 July, 2003

View this Special Issue

Open Access

Volume 18 |Article ID 464850 | https://doi.org/10.1155/2004/464850

Enrico Dainese, Annalaura Sabatucci, Roberto Minafra, Patrice Vachette, Edon Melloni, Ivo Cozzani, "Molecular events involved in the activation of calpain from human erythrocytes", Journal of Spectroscopy, vol. 18, Article ID 464850, 9 pages, 2004. https://doi.org/10.1155/2004/464850

Molecular events involved in the activation of calpain from human erythrocytes

Abstract

Calpains are intracellular cysteine endopeptidases that combine protease activity with a dependence on Ca2+ binding. Here we describe the conformational changes leading to the calpain activation, occurring in the enzyme purified from human erythrocytes, studied in solution using small angle X-ray scattering (SAXS). Addition of Ca2+ determines the formation of large soluble aggregates that can be dissociated either chelating these ions or adding cahotropic salts in solution. On the other side, our SAXS studies revealed that the addition of Ca2+ in the presence of inhibitors as E64 or leupeptin triggers a reversible conformational transition leading to a proper assembly of the active site without any aggregation or dissociation process. It is suggested that the observed conformational changes can be considered as the early step in the sequence of molecular events leading to calpain activation.

Copyright © 2004 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


More related articles

 PDF Download Citation Citation
 Order printed copiesOrder
Views71
Downloads285
Citations

Related articles

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.