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Spectroscopy
Volume 20, Issue 5-6, Pages 269-273
http://dx.doi.org/10.1155/2006/152816

Raman spectroscopic study of photosensitive damage to lysozyme structure sensitized by hypocrellin A

J. H. Zhou,1,3 X. H. Wu,1 S. H. Wei,1 X. T. Gu,1 Y. Y. Feng,1 X. S. Wang,2 and B. W. Zhang2

1Analysis & Testing Center, Nanjing Normal University, 210097 Nanjing, China
2Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100101, China
3Analysis & Detecting Center, Nanjing Normal University, 122 Ninghai Road, Nanjing, Jiangsu 210097, China

Copyright © 2006 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Laser Raman spectroscopy was used to detect the nature of the structural change in lysozyme sensitized by hypocrellin A (HA) at the molecular level. The results indicated that the orderly structure of lysozyme has been damaged by the active oxygen (1O2, O2−· and .OH, etc.) generated by HA, and cause the changes in H-bonds system of the main chain and the side chain of lysozyme.