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Volume 21, Issue 5-6, Pages 269-278

Investigation of diabetes-induced effect on apex of rat heart myocardium by using cluster analysis and neural network approach: An FTIR study

Neslihan Toyran,1 Feride Severcan,2 Mete Severcan,3 and Belma Turan4

1Department of Physiology, Faculty of Medicine, Baskent University, 06530 Ankara, Turkey
2Department of Biological Sciences, Middle East Technical University, 06531 Ankara, Turkey
3Electrical and Electronics Engineering Department, Middle East Technical University, 06531 Ankara, Turkey
4Department of Biophysics, School of Medicine, Ankara University, 06100 Ankara, Turkey

Copyright © 2007 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Diabetes mellitus (DM) is a progressive chronic disorder, which affects people belonging to all age groups of the population. This disease is accompanied by a greatly increased risk of cardiovascular death. In the present study, the effects of streptozotocin (STZ)-induced type 1 diabetes on apex myocardium of the rat heart have been investigated using Fourier Transform Infrared (FTIR) spectra. The cluster analysis has been applied to FTIR spectra to differentiate the diabetic samples from the normal controls. In addition, the protein secondary structures of diabetic and normal tissues were predicted by neural networks based on the amide I band of the FTIR spectra. The findings mainly suggest that 5 weeks of diabetes alters the lipid and protein profile of normal rat heart apex myocardium, which might have an important role in understanding the molecular mechanism of diabetes-related heart diseases.