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Volume 21 (2007), Issue 5-6, Pages 257-268

The structural stability of protein antigens adsorbed by aluminium hydroxide in comparison to the antigens in solutions

Yiwu Zheng,1,2 Xuxin Lai,1,2 Henrik Ipsen,1 Jørgen Nedergaard Larsen,1 Henning Løwenstein,1 Ib Søndergaard,2 and Susanne Jacobsen2

1ALK-ABELLO A/S, Hørsholm, Denmark
2Enzyme and Protein Chemistry, BioCentrum-DTU, Technical University of Denmark, Kgs. Lyngby, Denmark

Copyright © 2007 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


It is believed that antigens should be adsorbed onto adjuvants in vaccines. The adsorption-modified structure of antigens is important to understand the mechanism of adjuvants and vaccine immunogenicity. The structural stability of antigens is of major importance. The changes in structure can be induced by degradation and/or increase of storage temperature. In this study the structural stability of two model antigens, bovine serum albumin (BSA) and β-lactoglobulin (BLG) were compared when they were adsorbed onto aluminium hydroxide and when they were in solutions using Fourier transform infrared – attenuated total reflection (FTIR-ATR) spectroscopy. The structural stability of these two proteins was studied at different temperature and during storages. The present results showed that the structure of antigens can be stabilized by adsorption onto aluminium hydroxide. Non-adsorbed protein antigens present in vaccines may facilitate the degradation of the vaccine.