Journal of Spectroscopy

Journal of Spectroscopy / 2009 / Article

Open Access

Volume 23 |Article ID 456049 | 10 pages | https://doi.org/10.3233/SPE-2009-0389

Characterization of Bacillus subtilis sporulation and bacteriophage infection via FT-IR spectroscopy

Abstract

A comparative analysis on cultures from wild-type and sporulation mutants of Bacillus subtilis infected with pseudotemperate spore-converting bacteriophages was performed to discern differences in the chemical functionality of the cellular population. Variations in the cell surface chemistry of B. subtilis cultures were analyzed using attenuated total reflectance (ATR) Fourier transform infrared (FT-IR) spectroscopy of dried sample films on optical plates. Comparison of wild-type and phage infected cultures revealed an increase in the amount of aliphatic ester in the infected populations. Spectral analysis of cultures from bacteriophage infected sporulation mutants, spo0K and spo0J revealed varying levels of ester linkages.

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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