Journal of Spectroscopy

Journal of Spectroscopy / 2009 / Article

Open Access

Volume 23 |Article ID 605756 | https://doi.org/10.3233/SPE-2009-0370

Luca Nardo, Maria Bondani, Alessandra Andreoni, "Discrimination of the binding mode of DNA ligands by single-photon timing", Journal of Spectroscopy, vol. 23, Article ID 605756, 18 pages, 2009. https://doi.org/10.3233/SPE-2009-0370

Discrimination of the binding mode of DNA ligands by single-photon timing

Abstract

We perform time-correlated single-photon counting measurements with 30-ps resolution of the fluorescence emitted by the donor fluorophores of donor–acceptor pairs covalently labeling DNA synthetic oligonucleotides by using a non-commercial single-photon avalanche diode. The measurements allow us to precisely assess the fluorescence resonant energy transfer efficiency of the pairs in solutions containing the oligonucleotides and DNA–ligands, for different ratios of the DNA base-pair to ligand concentrations. A quantitative evaluation of the deformations of DNA double strands following the ligand binding is obtained, as the transfer efficiency is a steep function of the donor-to-acceptor distance. The results lead to an easy and cheap method to discriminate between the binding modes of minor groove and base intercalating ligands.

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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