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Volume 24 (2010), Issue 1-2, Pages 113-117

Raman spectroscopy methods for detecting and imaging supported lipid bilayers

Claire S. Sweetenham and Ioan Notingher

Nanoscience Group, School of Physics and Astronomy, University of Nottingham, University Park, Nottingham, UK

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We have developed a Raman microspectroscopy system optimised for studying supported lipid bilayers (SLB). This system combines the benefits of Raman spectroscopy with the high spatial resolution of confocal microscopy. Furthermore, the additional incorporation of an atomic force microscope (AFM) makes it possible to directly correlate chemical information with spatial features of samples at the nanoscale. We focus on the limits of this system for detecting a single SLB and imaging its microdomains, and employ surface-enhanced Raman spectroscopy (SERS) to improve the sensitivity achieved with Raman microspectroscopy.