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Volume 24 (2010), Issue 1-2, Pages 105-111

FTIR imaging and ATR-FT-Far-IR synchrotron spectroscopy of pig ear skin

Tanja M. Greve,1,2,4 Kristine B. Andersen,1 Ole F. Nielsen,2 and Anders Engdahl3

1Spectroscopy and Physical Chemistry, LEO Pharma A/S, Ballerup, Denmark
2Department of Chemistry, University of Copenhagen, Copenhagen, Denmark
3MAX-lab, Lund University, Lund, Sweden
4LEO Pharma A/S, Spectroscopy and Physical Chemistry,, Industriparken 55, DK-2750 Ballerup, Denmark

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


FTIR imaging was performed on pig ear skin samples cryo-sectioned perpendicular to the skin surface. The OH-stretch region revealed the distribution of water; the amide II band gave the protein distribution; the C═O stretch and C–H stretch regions showed the variation in lipids. Water and proteins were similarly distributed. Triglycerides were predominantly found in the deeper skin layers whereas free fatty acids and ceramides were more dominant in the upper layers. ATR-FT-Far-IR spectroscopy with synchrotron radiation was used on full thickness pig ear skin biopsies. The Far-IR spectra showed bands in the region from 100–150 cm–1 due to hydrogen bonded proteins and a band around 180 cm–1 arising from “free” water.