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Volume 24, Issue 3-4, Pages 333-338

Primary events in the photodissociation of oxyhemoglobin

Atsushi Yabushita1,5 and Takayoshi Kobayashi1,2,3,4

1Department of Electrophysics, National Chiao-Tung University, Hsichu, Taiwan
2ICORP, JST, Honcho, Kawaguchi, Saitama, Japan
3Department of Applied Physics and Chemistry and Institute for Laser Science, University of Electro-Communications, Chofu, Tokyo, Japan
4Institute of Laser Engineering, Osaka University, Suita, Osaka, Japan
5Department of Electrophysics, National Chiao-Tung University, Hsichu, 300, Taiwan

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The growing number of data on heme proteins in the ultrafast time domain have revealed complicated photophysics of the heme that are sensitive both to the structure of the protein and to the ligand species. In this work, ultrafast time-resolved pump–probe in visible spectral range was performed using an ultrashort visible laser pulse. Broad spectral width of visible laser pulse enabled us to observe the pump–probe signal with a broadband range. A broadband multi-channel detector array coupled to a multi-channel lock-in amplifier was used to obtain the pump–probe signals at all of the probe frequencies simultaneously. In the simultaneous measurement at many probe wavelengths, we could obtain ultrafast spectral change after the photo-excitation of oxy-hemoglobin only with a short measurement time, avoiding laser damage on the sample. The time constant of the primary process was determined for the first time. Ultrafast spectral change observed in the early delay time region directly shows ultrafast photo-dissociation process of oxy-hemoglobin.